搜索结果: 1-15 共查到“植物保护学 detection”相关记录31条 . 查询时间(0.134 秒)
Detection of candidate genes and development of KASP markers for Verticillium wilt resistance by combining genome wide association study, QTL seq and transcriptome sequencing in cotton
棉花 抗黄萎病性状 关联群体 遗传分离群体
2024/4/16
构建了棉花抗黄萎病性状的关联群体和遗传分离群体，联合利用全基因组关联分析(GWAS)、QTL- Seq和转录组测序开展了棉花抗黄萎病基因发掘与KASP标记开发研究,获得了5个主效抗黄萎病QTL的候选基因区间，开发出10个可以用于陆地棉抗病性标记辅助选择育种的KASP标记，同时鉴定出8个抗黄萎病候选基因，这些基因主要参与植物基础免疫反应及广谱抗病性，推测基础免疫反应相关基因在抗、感品种间的gDNA&...
The first detection of leafhopper Scaphoideus titanus Ball (Hemiptera, Cicadellidae) in Slovakia
American grapevine leafhopper occurrence phenology Flavescence doree
2015/7/28
Scaphoideus titanus Ball, the main vector of Flavescence dorée phytoplasma, was monitored in Slovakia during 2013 and 2014. The species was present in the vineyards of eastern Slovakia but it was rare...
Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR
CLRV SLRSV RT-PCR Xiphinema diversicaudatum
2015/3/3
one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The ...
The Production of Antiserum against Myrobalan Latent Ringspot Virus for Detection of the Virus using ELISA
nepovirus MLRSV purification immunisation of rabbits
2015/3/2
Myrobalan latent ringspot virus (MLRSV) was purified from the extract of infected Chenopodium quinoa Willd.,
using n-octanol, differential centrifugation, and saccharose gradient centrifugation. Ele...
Detection of Clavibacter michiganensis subsp. sepedonicus in daughter tubers of volunteer potato plants
potato Clavibacter michiganensis subsp. sepedonicus bacterial ring rot potato volunteer plants incidence of occurrence DAS ELISA Czech Republic
2015/3/1
Daughter tubers of volunteer potatoes were tested for their ability to maintain Clavibacter michiganensis subsp. sepedonicus (Cms). In different areas of the CR, volunteer potatoes were searched for i...
Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR
Ganoderma early diagnosis PCR ELISA integrated disease management
2015/2/6
Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymer...
Detection of phytoplasma ESFY in apricot trees using phloem and petioles
ESFY apricot PCR detectability symptoms
2015/2/2
ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which ...
PCR detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus
hop powdery mildew hop downy mildew internal transcribed spacers (ITS) PCR detection phylogenetic analysis
2015/2/2
Hop downy mildew (Pseudoperonospora humuli) and hop powdery mildew (Podosphaera macularis) are the most important pathogens of hop (Humulus lupulus). The early detection and identification of these pa...
Detection of Apple stem grooving virus in different tissues of apple trees throughout the year
Apple stem grooving virus tissue sampling RT-PCR ELISA reliability of detection
2015/1/30
The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all ti...
The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars
RT-PCR ASPV ASGV Apple stem pitting virus Apple stem grooving virus
2015/1/28
The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultiva...
Utilisation of immunochemical methods for detection of Colletotrichum spp. in strawberry
Colletotrichum acutatum strawberry pathogenicity polyclonal antibodies monoclonal antibodies PTA-ELISA immunoprint dot blot immunofluorescent microscopy
2015/1/28
Four polyclonal and two monoclonal antibodies were prepared and tested to detect a quarantine pathogen of strawberry – Colletotrichum acutatum. Only one of them, polyclonal IgG K91, was sensitive enou...
Sensitivity and specificity of monoclonal antibody Mn-Cs1 for detection and determination of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato
bacterial ring rot of potato Clavibacter michiganensis subsp sepedonicus DAS-ELISA
2015/1/28
Monoclonal antibody Mn-Cs1 with a high level of sensitivity and specificity for detection and determination of Clavibacter michiganensis subsp. sepedonicus was prepared. Strain C. m. subsp. sepedonicu...
he first detection of a phytoplasma from the 16SrV (Elm yellows) group in the mosaic leafhopper Orientus ishidae
Slovenia Flavescence dorée PCR
2014/11/25
In 2009 in Slovenia, an uncultivable cell-wall-less pathogenic bacterium of the class Mollicutes - a phytoplasma belonging to the 16SrV (Elm yellows) group was found in the mosaic leafhopper (Orientus...
Detection of unintended effects in genetically modified herbicide-tolerant (GMHT) rice in comparison with non-target phenotypic characteristics
Unintended effects safety assessment biosafety GM crops herbicide tolerance
2011/1/11
In the process of safety assessments of genetically modified (GM) crops, particular attention should be paid to their unintended effects. This study was focused on detection of unintended effects in g...
Simultaneous detection of three Potyvirus species from Cucurbitaceae crops by multiplex RT-PCR
Watermelon mosaic virus (WMV) Zucchini yellow mosaic virus (ZYMV) Papaya ringspot virus (PRSV) Multiplex RT-PCR
2009/3/17
A multiplex RT-PCR procedure was developed for the simultaneous detection of Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus (PRSV) from Cucurbitaceae cr...